datatype description: Sequencing analysis of RNA expression
rnaextract description: Rna shorter than 200 nt that has not been seperated based on Poly Adenalyation
readtype description: Single 36 nt reads
localization description: The fluid between the cells outer membrane and the nucleus
protocol description: This kind of library was made from RNA < 200 nucleotides that were pre-treated with Tobacco Acid Pyrophosphatase to remove any 5' caps that would preclude cloning. Hence, both capped and 5' monophosphate RNAs will be cloned.
localization description: Large membrane bound part of cell containing chromosomes and the bulk of the cell's DNA
cell description: skin fibroblast, "The line was established from skin taken from normal foreskin." - ATCC. (PMID: 9916803)
readtype description: Single 101 nt reads
protocol description: This kind of library was made from RNA < 200 nucleotides that was pre-treated sequentially with Calf Intestinal Alkaline Phosphatase followed by Tobacco Acid Pyrophosphatase. Hence, it will be enriched for capped RNAs.
protocol description: No special treatment or protocol applies
localization description: The part of the nucleus where ribosomal RNA is actively transcribed
localization description: Nuclear DNA and associated proteins