condition: Quiescence induced by serum depletion
condition: pre-senescence; 5 dys after RASG12V induction
protocol: BJ cells expressing hTERT and tamoxifen-inducible RASG12V were cultured in the presence of 10-7M 4-OHT-Tamoxifen for 5 days
condition: Senescence; 14 dys after RASG12V induction
protocol: BJ cells expressing hTERT and tamoxifen-inducible RASG12V were cultured in the presence of 10-7M 4-OHT-Tamoxifen for 14 days
condition: Transformed cells (induced by RASG12V in the background of stable p53 and p16INK4A kds)
protocol: BJ cells expressing hTERT, p16INK4A-KD, p53-KD and SV40 small T were retrovirally transduced with pBabe-puro-RASG12V