project	sample	experiment	run	read_count_as_reported_by_sra	reads_downloaded	proportion_of_reads_reported_by_sra_downloaded	paired_end	sra_misreported_paired_end	mapped_read_count	auc	sharq_beta_tissue	sharq_beta_cell_type	biosample_submission_date	biosample_publication_date	biosample_update_date	avg_read_length	geo_accession	bigwig_file	title	characteristics
SRP062617	SRS1040826	SRX1159817	SRR2175387	40159740	40159740	1	TRUE	FALSE	39575908	3962775312	NA	NA	2015-08-19T11:45:16.000	2015-09-30T01:27:33.593	2015-09-30T01:31:42.550	202	GSM1857484	SRR2175387.bw	Glial_Progenitors	c("growth protocol: Normal, non-neoplastic cells were derived from patient tissue specimens of neurosurgical resection in accordance with a Cleveland Clinic Institutional Review Board-approved protocol. Informed consent was obtained by the tissue bank, which provided de-identified excess tissue to the laboratory immediately following surgical resection. Specimens used for cell culture were dissociated with a Papain dissociation kit (Worthington). Cells were cultured adherently in media containing 50% Neurobasal medium (Gibco) and 50% Dulbecco's modified Eagle medium (DMEM) with B27 (without vitamin A, Invitrogen, basic fibroblast growth factor (10 ng/ml), epidermal growth factor (10 ng/ml), sodium pyruvate and L-glutamine, and 5% FBS. All cultured cells were used within five passages of dissociation.", 
"cell characterization: Single cells were sorted using anti-A2B5 MicroBeads (130-093-388, Miltenyi Biotec) according to manufacturer’s protocol. In brief, live cells were incubated first with FcR Blocking Reagent (Miltenyi) for 10 mins then A2B5 antibody for 15 min at 4oC (2.5 μg A2B5 antibody per million cells). Cells positive for A2B5 were double enriched by passing through magnetic field (MACS Separator) twice. Both A2B5 positive and negative fractions were collected, lysed with TRIzol (Invitrogen) for RNA isolation. A population of A2B5+ cells were plated and allowed to attach overnight before fixing and staining with standard immunofluorescence techniques. A2B5+ cells stained positive for GFAP (DAKO, 1:5000) and Nestin (DSHB, 1:200)."
)
SRP062617	SRS1040827	SRX1159818	SRR2175389	19481736	19481736	1	TRUE	FALSE	19005910	1885294964	NA	NA	2015-08-19T11:46:19.000	2015-09-30T01:27:35.037	2015-09-30T01:31:42.083	202	GSM1857485	SRR2175389.bw	hES_OPCs	c("growth protocol: Human OPCs were differentiated from the human embryonic stem cell line H7 over a 154 day protocol (NIH Human Embryonic Stem Cell Registry WA07; NIH Approval Number: NIHhESC-10-0061) as previously demonstrated (Hu et al., 2009; Wang et al., 2013).", "cell characterization: hESC-derived OPCs were characterized by co-staining of Sox10 (R&D Systems,AF2864; 1:100) and Olig2 (Millipore,AB9610 1:500).")
SRP062617	SRS1040827	SRX1159818	SRR2175390	19199760	19199760	1	TRUE	FALSE	18729534	1858178617	NA	NA	2015-08-19T11:46:19.000	2015-09-30T01:27:35.037	2015-09-30T01:31:42.083	202	GSM1857485	SRR2175390.bw	hES_OPCs	c("growth protocol: Human OPCs were differentiated from the human embryonic stem cell line H7 over a 154 day protocol (NIH Human Embryonic Stem Cell Registry WA07; NIH Approval Number: NIHhESC-10-0061) as previously demonstrated (Hu et al., 2009; Wang et al., 2013).", "cell characterization: hESC-derived OPCs were characterized by co-staining of Sox10 (R&D Systems,AF2864; 1:100) and Olig2 (Millipore,AB9610 1:500).")
SRP062617	SRS1040828	SRX1159816	SRR2175385	46879460	46879460	1	TRUE	FALSE	43163133	4303980368	NA	NA	2015-08-19T11:45:15.000	2015-09-30T01:27:31.220	2015-09-30T01:31:41.830	202	GSM1857483	SRR2175385.bw	p0-IN528_GBM-CSCs	c("growth protocol: IN528 cells from a primary patient derived GBM tumor model were injected subcutaneously into the flank of a immunocomprimised athymic nude mouse and allowed to grow into a tumor.", "cell characterization: Cells were dissociated from the tumor and sorted on CD133 to collect the cancer stem cell population.")
SRP062617	SRS1040828	SRX1159816	SRR2175386	46335502	46335502	1	TRUE	FALSE	42651365	4253043450	NA	NA	2015-08-19T11:45:15.000	2015-09-30T01:27:31.220	2015-09-30T01:31:41.830	202	GSM1857483	SRR2175386.bw	p0-IN528_GBM-CSCs	c("growth protocol: IN528 cells from a primary patient derived GBM tumor model were injected subcutaneously into the flank of a immunocomprimised athymic nude mouse and allowed to grow into a tumor.", "cell characterization: Cells were dissociated from the tumor and sorted on CD133 to collect the cancer stem cell population.")
SRP062617	SRS1040826	SRX1159817	SRR2175388	39594644	0	0	TRUE	FALSE	0	NA	NA	NA	2015-08-19T11:45:16.000	2015-09-30T01:27:33.593	2015-09-30T01:31:42.550	202	GSM1857484	NA	Glial_Progenitors	c("growth protocol: Normal, non-neoplastic cells were derived from patient tissue specimens of neurosurgical resection in accordance with a Cleveland Clinic Institutional Review Board-approved protocol. Informed consent was obtained by the tissue bank, which provided de-identified excess tissue to the laboratory immediately following surgical resection. Specimens used for cell culture were dissociated with a Papain dissociation kit (Worthington). Cells were cultured adherently in media containing 50% Neurobasal medium (Gibco) and 50% Dulbecco's modified Eagle medium (DMEM) with B27 (without vitamin A, Invitrogen, basic fibroblast growth factor (10 ng/ml), epidermal growth factor (10 ng/ml), sodium pyruvate and L-glutamine, and 5% FBS. All cultured cells were used within five passages of dissociation.", 
"cell characterization: Single cells were sorted using anti-A2B5 MicroBeads (130-093-388, Miltenyi Biotec) according to manufacturer’s protocol. In brief, live cells were incubated first with FcR Blocking Reagent (Miltenyi) for 10 mins then A2B5 antibody for 15 min at 4oC (2.5 μg A2B5 antibody per million cells). Cells positive for A2B5 were double enriched by passing through magnetic field (MACS Separator) twice. Both A2B5 positive and negative fractions were collected, lysed with TRIzol (Invitrogen) for RNA isolation. A population of A2B5+ cells were plated and allowed to attach overnight before fixing and staining with standard immunofluorescence techniques. A2B5+ cells stained positive for GFAP (DAKO, 1:5000) and Nestin (DSHB, 1:200)."
)