c("growth protocol: Normal, non-neoplastic cells were derived from patient tissue specimens of neurosurgical resection in accordance with a Cleveland Clinic Institutional Review Board-approved protocol. Informed consent was obtained by the tissue bank, which provided de-identified excess tissue to the laboratory immediately following surgical resection. Specimens used for cell culture were dissociated with a Papain dissociation kit (Worthington). Cells were cultured adherently in media containing 50% Neurobasal medium (Gibco) and 50% Dulbecco's modified Eagle medium (DMEM) with B27 (without vitamin A, Invitrogen, basic fibroblast growth factor (10 ng/ml), epidermal growth factor (10 ng/ml), sodium pyruvate and L-glutamine, and 5% FBS. All cultured cells were used within five passages of dissociation.",
c("growth protocol: Human OPCs were differentiated from the human embryonic stem cell line H7 over a 154 day protocol (NIH Human Embryonic Stem Cell Registry WA07; NIH Approval Number: NIHhESC-10-0061) as previously demonstrated (Hu et al., 2009; Wang et al., 2013).", "cell characterization: hESC-derived OPCs were characterized by co-staining of Sox10 (R&D Systems,AF2864; 1:100) and Olig2 (Millipore,AB9610 1:500).")
c("growth protocol: Human OPCs were differentiated from the human embryonic stem cell line H7 over a 154 day protocol (NIH Human Embryonic Stem Cell Registry WA07; NIH Approval Number: NIHhESC-10-0061) as previously demonstrated (Hu et al., 2009; Wang et al., 2013).", "cell characterization: hESC-derived OPCs were characterized by co-staining of Sox10 (R&D Systems,AF2864; 1:100) and Olig2 (Millipore,AB9610 1:500).")
c("growth protocol: IN528 cells from a primary patient derived GBM tumor model were injected subcutaneously into the flank of a immunocomprimised athymic nude mouse and allowed to grow into a tumor.", "cell characterization: Cells were dissociated from the tumor and sorted on CD133 to collect the cancer stem cell population.")
c("growth protocol: IN528 cells from a primary patient derived GBM tumor model were injected subcutaneously into the flank of a immunocomprimised athymic nude mouse and allowed to grow into a tumor.", "cell characterization: Cells were dissociated from the tumor and sorted on CD133 to collect the cancer stem cell population.")