mRNA-seq from M cells, pretreated with cycloheximide_2
mRNA-seq from M cells, pretreated with cycloheximide_1
mRNA-seq from G2 cells, pretreated with cycloheximide_2
mRNA-seq from G1 cells, pretreated with cycloheximide_2
mRNA-seq from G2 cells, pretreated with cycloheximide_1
mRNA-seq from G1 cells, pretreated with cycloheximide_1

cell type: RPE-1 cells immortalized with hTERT
cell synchronization: Cells were treated with 6 micromolar RO-3306 for 18 hr. RO-3306 was then removed and mitotic cells were harvested 45 min later through mitotic shake-off
cell cycle timepoint: M
drug pre-treatment: cells were pretreated with cycloheximide
cell type: RPE-1 cells immortalized with hTERT
cell synchronization: Cells were treated with 6 micromolar RO-3306 for 18 hr. RO-3306 was then removed and mitotic cells were harvested 45 min later through mitotic shake-off
cell cycle timepoint: M
drug pre-treatment: cells were pretreated with cycloheximide
cell type: RPE-1 cells immortalized with hTERT
cell synchronization: Cells were treated with 6 micromolar RO-3306 for 18 hr and harvested
cell cycle timepoint: G2
drug pre-treatment: cells were pretreated with cycloheximide
cell type: RPE-1 cells immortalized with hTERT
cell synchronization: Cells were treated with 6 micromolar RO-3306 for 18 hr. RO-3306 was then removed and mitotic cells were collected 45 min later through mitotic shake-off and replated to allow normal mitotic exit. 3hr later G1 cells were harvested.
cell cycle timepoint: G1
drug pre-treatment: cells were pretreated with cycloheximide
cell type: RPE-1 cells immortalized with hTERT
cell synchronization: Cells were treated with 6 micromolar RO-3306 for 18 hr and harvested
cell cycle timepoint: G2
drug pre-treatment: cells were pretreated with cycloheximide
cell type: RPE-1 cells immortalized with hTERT
cell synchronization: Cells were treated with 6 micromolar RO-3306 for 18 hr. RO-3306 was then removed and mitotic cells were collected 45 min later through mitotic shake-off and replated to allow normal mitotic exit. 3hr later G1 cells were harvested.
cell cycle timepoint: G1
drug pre-treatment: cells were pretreated with cycloheximide