3719:115786	ERR1096225	ERP012914	ERS943748	ERX1175759	ERA526606	LUDWIG INSTITUTE FOR CANCER RESEARCH	European Nucleotide Archive	Transcriptional profiling of HAP1 kinase-knockout cells upon stimulation with polypeptides and small molecules	This dataset is a systematic study of transcriptional profiles of HAP1 cells under environmental and genetic perturbations. Samples were prepared using wildtype HAP1 cells and HAP-derived cell lines that included CRISPR/Cas9-induced gene knockout in tyrosine kinases. Cells were cultured in reduced serum conditions for 16h, then stimulated with a polypeptide or small molecule for 6 hours. Cells were harvested and extracted RNA was subjected to RNA-seq library preparation in 96-well plates using the QuantSeq protocol. Sequencing was performed on an Illumina HiSeq 2000 system following a T-fill reaction, with 48 samples multiplexed on each lane.		Illumina HiSeq 2000 sequencing; Shallow transcriptomic profiling of HAP1 cells (EPHA6_BMP2_R1)	HAP1 EPHA6-KO cells, stimulated with BMP2, replicate R1		KiCube_Library_EPHA6_BMP2_R1	RNA-Seq	TRANSCRIPTOMIC	Oligo-dT	single			Quant-Seq with T-fill	Illumina HiSeq 2000	Alias;;KiCube_Sample_EPHA6_BMP2_R1|Column.96well;;11|Description;;HAP1 EPHA6-KO cells, stimulated with BMP2, replicate R1|ENA checklist;;ERC000011|ERCC;;no|ExpectedSig;;WT_BMP2|Group;;EPHA6_BMP2|INSDC center alias;;LUDWIG INSTITUTE FOR CANCER RESEARCH|INSDC center name;;LUDWIG INSTITUTE FOR CANCER RESEARCH|INSDC first public;;2016-05-19T17:00:53Z|INSDC last update;;2018-03-09T09:26:06Z|INSDC status;;public|Lane;;BG_Plex019|Library.concentration;;5.44|Plate.96well;;5|RefGroup;;EPHA6_None|RNA.concentration;;246.2|Row.96well;;E|Sample Name;;ERS943748|Sample;;EPHA6_BMP2_R1|SRA accession;;ERS943748|Stimulus;;BMP2|Title;;HAP1 EPHA6-KO cells, stimulated with BMP2, replicate R1|X260.230;;2.3|X260.280;;2.01			SAMEA3636599	KiCube_Sample_EPHA6_BMP2_R1	139185550	2783711	2016-05-20 15:10:34	173254725	139185550	2783711	1	2783711	index:0,count:2783711,average:50,stdev:0	KiCube_Run_EPHA6_BMP2_R1	LUDWIG INSTITUTE FOR CANCER RESEARCH		CE-M-M-, RESEARCH CENTER FOR MOLECULAR MEDICINE OF THE AUSTRIAN ACADEMY OF SCIENCES	13.02	4.47	0.07	122420843	168066259	101370663	143208880	137.29	141.27	0	0	0	0	0	0	62.92	76.07	3656632	1573961	3656632	1573961	68.09	69.35	3656632	1703142	3656632	1434950	5171028	4.22	2.30	0	15.53	0	0.59	0	0.15	0	0.00	0	9.40	0	2501411	0	50	0	48.99	0	1.59	0	0.01	0	1.28	0	0.00	0	501.07	0	0.63	0	64074	0	2783711	0	432189	0	16511	0	4231	0	0	0	261558	0	16	0	0	0	292	0	23884	0	1832	0	26024	0	74.33	0	2069222	0	4079	26243	6.433684726649	2783711.0	2501411.0	64074.0	432189.0	16511.0	4231.0	0.0	261558.0	2069222.0	89.9	2.3	15.5	0.6	0.2	0.0	9.4	74.3